induction of drug resistance in plasmodium falciparum: an intermittent drug exposure method
نویسندگان
چکیده
the production of experimentally induced drug resistance in the laboratory provides valuable opportunities for investigators to study the nature and genetics of drug resistance mechanisms to a given agent, patterns of cross resistance and the mode of action of drugs. at the beginning the continuous drug exposure was chosen as a standard procedure to produce drug— resistant strains of p. falciparum,.but later on some other methods were also applied. an intermittent drug exposure method as a novel procedure has been introduced in this study. intermittent exposure of chloroquine resistant kl and chloroquine sensitive t9.96 strains of p. falciparum to halofantrine culminated in a relatively rapid reduction in sensitivity to the drug. the response of halofantrifle - resistnat k1hf and t9.96 strains and parent parasites to halofantrifle, inefloquine, quinine and chloroquine was determined. the results indicated that the effectiveness of halofantrine to k1hf and t9.96hf strains decreased 9 and 3 folds respectively, compared to the parent parasites. cross -resistance occurred among halofantrine. mefloquine and quinine. halofantrine resistance was associated with enhanced chloroquine sensitivity in the strain derived from chloroquine - resistant k1 strain, hut not in the strain derived from chloroquine - sensitive t9.96 parasites.
منابع مشابه
Variations in frequencies of drug resistance in Plasmodium falciparum.
Continual exposure of malarial parasite populations to different drugs may have selected not only for resistance to individual drugs but also for genetic traits that favor initiation of resistance to novel unrelated antimalarials. To test this hypothesis, different Plasmodium falciparum clones having varying numbers of preexisting resistance mechanisms were treated with two new antimalarial age...
متن کاملGenetic alteration in drug resistance markers of Plasmodium falciparum.
Plasmodium falciparum shows plasticity in its genome. For its survival it can delete certain genes (or portions) if not needed for its growth and has the capability to regulate its genes under various stages of its life cycle as well as under unfavourable environmental conditions. Parasite shows enormous amount of antigenic variation under immune pressure leading to the emergence of vaccine res...
متن کاملRESISTANCE OF PLASMODIUM FALCIPARUM TO CHLOROQUINE IN SOUTH EASTERN IRAN
In vivo and in vitro assessments of the response of P. falciparum to chloroquine using WHO standard kits and techniques were carried out in I ran Shahr, Sistan and Baluchestan province of Iran in 1985. In the in vivo assessment, 24 malaria patients treated with chloroquine (25mg/kg over three days) were followed up for one to four weeks. The mean parasite clearance time was 4.3 days and in...
متن کاملHuman genetic variation is associated with Plasmodium falciparum drug resistance.
One approach to investigate if human genetic variation influences the selection of Plasmodium falciparum drug resistance is to compare the frequency of resistant infections among human populations differing in their genetic background and living in the same epidemiological context. A further complementary approach consists in comparing drug resistance among subjects differing for genes involved...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of public healthجلد ۲۷، شماره ۱-۲، صفحات ۴۳-۶۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023